Electroporation:Article Title: Chromatin-mediated anticipatory control of type I interferon production in plasmacytoid dendritic cells.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Goat anti-lamin B (M-20) Santa Cruz Biotechnology Cat# sc-6217; RRID:AB_648158 Rabbit anti-SMC3 mAb (D47B5) Cell Signaling Technology Cat# 5696; RRID:AB_10705575 AlexaFluor 488 goat anti-rabbit IgG (H+L) Thermo Fisher Scientific Cat# A-11034; RRID:AB_2576217 Rabbit anti-β-actin (RM112) Thermo Fisher Scientific Cat# MA5-33078; RRID:AB_2810170 Bacterial and virus strains SARS-CoV-2 MA10 BEI Resources NR-55329 Sendai virus (Cantell strain) Charles River (c/o D. Levy) N/A Chemicals, peptides, and recombinant proteins β-Estradiol Sigma-Aldrich Cat# E2758 Alt-R S.p. .. Cas9 Nuclease V3 IDT Cat# 1081058 Alt-R Cas9 Electroporation Enhancer IDT Cat# 1075915 (Z)-4-Hydroxytamoxifen Sigma-Aldrich Cat# H7904 Recombinant Mouse GM-CSF (carrier free) BioLegend Cat# 576304 Recombinant Mouse IL-4 (carrier free) BioLegend Cat# 574304 Collagenase D from Clostridium histolyticum Roche Cat# 11088858001 Pronase from Streptomyces griseus Millipore Sigma Cat# 10165921001 Amphotericin B solution Millipore Sigma Cat# A2942 Liberase TL Roche Cat# 5401020001 Bovine Serum Albumin, Powder Millipore Sigma Cat# A9647 Deoxyribonuclease I (DNase I) from bovine pancreas Roche Cat# 11284932001 RBC Lysis Buffer BioLegend Cat# 420301 Poly(I:C) high molecular weight InvivoGen Cat# tlrl-pic CpG-A/ODN 2216 InvivoGen Cat# tlrl-2216-1 DOTAP chloride MedChemExpress Cat# HY-112754A Concanavalin A from Canavalia ensiformis Millipore Sigma Cat# L7647, C2010 Paraformaldehyde Aqueous Solution, EM Grade Electron Microscopy Sciences Cat# 15710 Triton X-100 Thermo Fisher Scientific Cat# 85111 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific Cat# 89900 Halt Protease Inhibitor Cocktail Thermo Fisher Scientific Cat# 78429 Halt Phosphatase Inhibitor Cocktail Thermo Fisher Scientific Cat# 78420 NuPAGE LDS Sample Buffer Thermo Fisher Scientific Cat# NP0007 NuPAGE Sample Reducing Agent Thermo Fisher Scientific Cat# NP0009 TRIzol Reagent Themo Fisher Scientific Cat# 15596018 Digitonin Thermo Fisher Scientific Cat# BN2006 Trizma Hydrochloride Solution, pH 7.4 Millipore Sigma Cat# T2194 Sodium Chloride Solution, 5 M Millipore Sigma Cat# 59222C Magnesium Chloride Solution, 1M Millipore Sigma Cat# M1028 IGEPAL CA-630 Millipore Sigma Cat# I8896 Protector RNase inhibitor Millipore Sigma Cat# 3335402001 DL-Dithiothreitol (DTT) solution, 1M Millipore Sigma Cat# 646563 Bovine Serum Albumin Fraction V Solution Thermo Fisher Scientific Cat# 15260037 10% Tween 20 Bio-Rad Cat# 1662404 Critical commercial assays Venor GeM Mycoplasma PCR-Based Detection Kit Sigma-Aldrich Cat# MP0025 SF Cell Line 4D-Nucleofector X Kit L Lonza Cat# V4XC-2012 KT Streptavidin microbeads Miltenyi Biotec Cat# 130-048-101 LD columns Miltenyi Biotec Cat# 130-042-901 (Continued on next page) Immunity 59, 270–287.e1–e11, February 10, 2026 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Combined snRNA-Seq and snATAC-Seq primary splenic pDCs Pucella et al. 72 GEO: GSE252189 Experimental models: Cell lines Mouse: L929 ATCC Cat# CCL-1, RRID:CVCL_0462 Mouse: B16-Flt3L Mach et al. 82 N/A Mouse: HoxB8-FL Redecke et al. 83 N/A Mouse: Pmi28 c/o C. Cardoso N/A African green monkey: Vero E6 ATCC Cat# CRL1586; RRID: CVCL_0574 Experimental models: Organisms/strains Mouse: Smc3 fl : B6(SJL)Smc3 tm1c(EUCOMM)Wtsi /J The Jackson Laboratory RRID: IMSR_JAX:030559 Mouse: Rosa26 CreERT2 Dr. Thomas Ludwig, Ohio State University N/A Mouse: Ifn ΔTB2 This paper N/A Mouse: Ifn ΔTB2/TB4 This paper N/A Mouse: Ifn ΔTB2/TB3/TB4 This paper N/A Mouse: Irf8 -/- : B6(Cg)-Irf8 tm1.2Hm /J The Jackson Laboratory RRID:IMSR_JAX:018298 Mouse: Ctrl for snRNA-Seq/snATAC-Seq: C57BL/6-Tg(CAG-EGFP)131Osb/LeySopJ The Jackson Laboratory RRID:IMSR_JAX:006567 Mouse: C57BL/6: C57BL/6NTac Taconic Biosciences Model# B6-F Mouse: BALB/c: BALB/cAnNTac Taconic Biosciences Model# BALB-M Oligonucleotides sgRNAs targeting IFN-I TB in mouse germline, see Table S6 This paper N/A sgRNAs for targeting in HoxB8-FL cells, see Table S6 This paper N/A Primers for genotyping of Ifn ΔTB2 , Ifn ΔTB3 , and Ifn ΔTB4 alleles, see Table S6 This paper N/A RT-qPCR primers for Ifn subtype quantification, see Table S6 This paper N/A Alt-R CRISPR-Cas9 Negative Control crRNA #1 IDT Cat# 1072544 Alt-R CRISPR-Cas9 tracrRNA IDT Cat# 1072532 BAC clones RP23-64G11, RP23-139P14 and RP23-360M23 BACPAC Resources N/A Software and algorithms FlowJo v10 Tree Star RRID: SCR_008520 GraphPad Prism 10 Graphpad Inc RRID: SCR_002798 ExAtlas https://kolab.elixirgensci.com/exatlas/ N/A Heatmapper http://www.heatmapper.ca/expression/ RRID:SCR_016974 IGV https://igv.org/ RRID:SCR_011793 Resgen https://resgen.io/ N/A ImageJ https://imagej.net/ij/ RRID:SCR_003070 bcl2fastq Illumina RRID:SCR_015058 Seq-N-Slide https://igordot.github.io/sns/ RRID:SCR_021752 Trimmomatic http://www.usadellab.org/cms/ ?page=trimmomatic RRID:SCR_011848 STAR aligner https://github.com/alexdobin/STAR RRID:SCR_004463 featureCounts https://subread.sourceforge.net/ featureCounts.html RRID:SCR_012919 DESeq2 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html RRID:SCR_015687 (Continued on next page) Immunity 59, 270–287.e1–e11, February 10, 2026 e4
Recombinant:Article Title: Chromatin-mediated anticipatory control of type I interferon production in plasmacytoid dendritic cells.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Goat anti-lamin B (M-20) Santa Cruz Biotechnology Cat# sc-6217; RRID:AB_648158 Rabbit anti-SMC3 mAb (D47B5) Cell Signaling Technology Cat# 5696; RRID:AB_10705575 AlexaFluor 488 goat anti-rabbit IgG (H+L) Thermo Fisher Scientific Cat# A-11034; RRID:AB_2576217 Rabbit anti-β-actin (RM112) Thermo Fisher Scientific Cat# MA5-33078; RRID:AB_2810170 Bacterial and virus strains SARS-CoV-2 MA10 BEI Resources NR-55329 Sendai virus (Cantell strain) Charles River (c/o D. Levy) N/A Chemicals, peptides, and recombinant proteins β-Estradiol Sigma-Aldrich Cat# E2758 Alt-R S.p. .. Cas9 Nuclease V3 IDT Cat# 1081058 Alt-R Cas9 Electroporation Enhancer IDT Cat# 1075915 (Z)-4-Hydroxytamoxifen Sigma-Aldrich Cat# H7904 Recombinant Mouse GM-CSF (carrier free) BioLegend Cat# 576304 Recombinant Mouse IL-4 (carrier free) BioLegend Cat# 574304 Collagenase D from Clostridium histolyticum Roche Cat# 11088858001 Pronase from Streptomyces griseus Millipore Sigma Cat# 10165921001 Amphotericin B solution Millipore Sigma Cat# A2942 Liberase TL Roche Cat# 5401020001 Bovine Serum Albumin, Powder Millipore Sigma Cat# A9647 Deoxyribonuclease I (DNase I) from bovine pancreas Roche Cat# 11284932001 RBC Lysis Buffer BioLegend Cat# 420301 Poly(I:C) high molecular weight InvivoGen Cat# tlrl-pic CpG-A/ODN 2216 InvivoGen Cat# tlrl-2216-1 DOTAP chloride MedChemExpress Cat# HY-112754A Concanavalin A from Canavalia ensiformis Millipore Sigma Cat# L7647, C2010 Paraformaldehyde Aqueous Solution, EM Grade Electron Microscopy Sciences Cat# 15710 Triton X-100 Thermo Fisher Scientific Cat# 85111 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific Cat# 89900 Halt Protease Inhibitor Cocktail Thermo Fisher Scientific Cat# 78429 Halt Phosphatase Inhibitor Cocktail Thermo Fisher Scientific Cat# 78420 NuPAGE LDS Sample Buffer Thermo Fisher Scientific Cat# NP0007 NuPAGE Sample Reducing Agent Thermo Fisher Scientific Cat# NP0009 TRIzol Reagent Themo Fisher Scientific Cat# 15596018 Digitonin Thermo Fisher Scientific Cat# BN2006 Trizma Hydrochloride Solution, pH 7.4 Millipore Sigma Cat# T2194 Sodium Chloride Solution, 5 M Millipore Sigma Cat# 59222C Magnesium Chloride Solution, 1M Millipore Sigma Cat# M1028 IGEPAL CA-630 Millipore Sigma Cat# I8896 Protector RNase inhibitor Millipore Sigma Cat# 3335402001 DL-Dithiothreitol (DTT) solution, 1M Millipore Sigma Cat# 646563 Bovine Serum Albumin Fraction V Solution Thermo Fisher Scientific Cat# 15260037 10% Tween 20 Bio-Rad Cat# 1662404 Critical commercial assays Venor GeM Mycoplasma PCR-Based Detection Kit Sigma-Aldrich Cat# MP0025 SF Cell Line 4D-Nucleofector X Kit L Lonza Cat# V4XC-2012 KT Streptavidin microbeads Miltenyi Biotec Cat# 130-048-101 LD columns Miltenyi Biotec Cat# 130-042-901 (Continued on next page) Immunity 59, 270–287.e1–e11, February 10, 2026 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Combined snRNA-Seq and snATAC-Seq primary splenic pDCs Pucella et al. 72 GEO: GSE252189 Experimental models: Cell lines Mouse: L929 ATCC Cat# CCL-1, RRID:CVCL_0462 Mouse: B16-Flt3L Mach et al. 82 N/A Mouse: HoxB8-FL Redecke et al. 83 N/A Mouse: Pmi28 c/o C. Cardoso N/A African green monkey: Vero E6 ATCC Cat# CRL1586; RRID: CVCL_0574 Experimental models: Organisms/strains Mouse: Smc3 fl : B6(SJL)Smc3 tm1c(EUCOMM)Wtsi /J The Jackson Laboratory RRID: IMSR_JAX:030559 Mouse: Rosa26 CreERT2 Dr. Thomas Ludwig, Ohio State University N/A Mouse: Ifn ΔTB2 This paper N/A Mouse: Ifn ΔTB2/TB4 This paper N/A Mouse: Ifn ΔTB2/TB3/TB4 This paper N/A Mouse: Irf8 -/- : B6(Cg)-Irf8 tm1.2Hm /J The Jackson Laboratory RRID:IMSR_JAX:018298 Mouse: Ctrl for snRNA-Seq/snATAC-Seq: C57BL/6-Tg(CAG-EGFP)131Osb/LeySopJ The Jackson Laboratory RRID:IMSR_JAX:006567 Mouse: C57BL/6: C57BL/6NTac Taconic Biosciences Model# B6-F Mouse: BALB/c: BALB/cAnNTac Taconic Biosciences Model# BALB-M Oligonucleotides sgRNAs targeting IFN-I TB in mouse germline, see Table S6 This paper N/A sgRNAs for targeting in HoxB8-FL cells, see Table S6 This paper N/A Primers for genotyping of Ifn ΔTB2 , Ifn ΔTB3 , and Ifn ΔTB4 alleles, see Table S6 This paper N/A RT-qPCR primers for Ifn subtype quantification, see Table S6 This paper N/A Alt-R CRISPR-Cas9 Negative Control crRNA #1 IDT Cat# 1072544 Alt-R CRISPR-Cas9 tracrRNA IDT Cat# 1072532 BAC clones RP23-64G11, RP23-139P14 and RP23-360M23 BACPAC Resources N/A Software and algorithms FlowJo v10 Tree Star RRID: SCR_008520 GraphPad Prism 10 Graphpad Inc RRID: SCR_002798 ExAtlas https://kolab.elixirgensci.com/exatlas/ N/A Heatmapper http://www.heatmapper.ca/expression/ RRID:SCR_016974 IGV https://igv.org/ RRID:SCR_011793 Resgen https://resgen.io/ N/A ImageJ https://imagej.net/ij/ RRID:SCR_003070 bcl2fastq Illumina RRID:SCR_015058 Seq-N-Slide https://igordot.github.io/sns/ RRID:SCR_021752 Trimmomatic http://www.usadellab.org/cms/ ?page=trimmomatic RRID:SCR_011848 STAR aligner https://github.com/alexdobin/STAR RRID:SCR_004463 featureCounts https://subread.sourceforge.net/ featureCounts.html RRID:SCR_012919 DESeq2 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html RRID:SCR_015687 (Continued on next page) Immunity 59, 270–287.e1–e11, February 10, 2026 e4
Red Blood Cell Lysis:Article Title: Chromatin-mediated anticipatory control of type I interferon production in plasmacytoid dendritic cells.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Goat anti-lamin B (M-20) Santa Cruz Biotechnology Cat# sc-6217; RRID:AB_648158 Rabbit anti-SMC3 mAb (D47B5) Cell Signaling Technology Cat# 5696; RRID:AB_10705575 AlexaFluor 488 goat anti-rabbit IgG (H+L) Thermo Fisher Scientific Cat# A-11034; RRID:AB_2576217 Rabbit anti-β-actin (RM112) Thermo Fisher Scientific Cat# MA5-33078; RRID:AB_2810170 Bacterial and virus strains SARS-CoV-2 MA10 BEI Resources NR-55329 Sendai virus (Cantell strain) Charles River (c/o D. Levy) N/A Chemicals, peptides, and recombinant proteins β-Estradiol Sigma-Aldrich Cat# E2758 Alt-R S.p. .. Cas9 Nuclease V3 IDT Cat# 1081058 Alt-R Cas9 Electroporation Enhancer IDT Cat# 1075915 (Z)-4-Hydroxytamoxifen Sigma-Aldrich Cat# H7904 Recombinant Mouse GM-CSF (carrier free) BioLegend Cat# 576304 Recombinant Mouse IL-4 (carrier free) BioLegend Cat# 574304 Collagenase D from Clostridium histolyticum Roche Cat# 11088858001 Pronase from Streptomyces griseus Millipore Sigma Cat# 10165921001 Amphotericin B solution Millipore Sigma Cat# A2942 Liberase TL Roche Cat# 5401020001 Bovine Serum Albumin, Powder Millipore Sigma Cat# A9647 Deoxyribonuclease I (DNase I) from bovine pancreas Roche Cat# 11284932001 RBC Lysis Buffer BioLegend Cat# 420301 Poly(I:C) high molecular weight InvivoGen Cat# tlrl-pic CpG-A/ODN 2216 InvivoGen Cat# tlrl-2216-1 DOTAP chloride MedChemExpress Cat# HY-112754A Concanavalin A from Canavalia ensiformis Millipore Sigma Cat# L7647, C2010 Paraformaldehyde Aqueous Solution, EM Grade Electron Microscopy Sciences Cat# 15710 Triton X-100 Thermo Fisher Scientific Cat# 85111 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific Cat# 89900 Halt Protease Inhibitor Cocktail Thermo Fisher Scientific Cat# 78429 Halt Phosphatase Inhibitor Cocktail Thermo Fisher Scientific Cat# 78420 NuPAGE LDS Sample Buffer Thermo Fisher Scientific Cat# NP0007 NuPAGE Sample Reducing Agent Thermo Fisher Scientific Cat# NP0009 TRIzol Reagent Themo Fisher Scientific Cat# 15596018 Digitonin Thermo Fisher Scientific Cat# BN2006 Trizma Hydrochloride Solution, pH 7.4 Millipore Sigma Cat# T2194 Sodium Chloride Solution, 5 M Millipore Sigma Cat# 59222C Magnesium Chloride Solution, 1M Millipore Sigma Cat# M1028 IGEPAL CA-630 Millipore Sigma Cat# I8896 Protector RNase inhibitor Millipore Sigma Cat# 3335402001 DL-Dithiothreitol (DTT) solution, 1M Millipore Sigma Cat# 646563 Bovine Serum Albumin Fraction V Solution Thermo Fisher Scientific Cat# 15260037 10% Tween 20 Bio-Rad Cat# 1662404 Critical commercial assays Venor GeM Mycoplasma PCR-Based Detection Kit Sigma-Aldrich Cat# MP0025 SF Cell Line 4D-Nucleofector X Kit L Lonza Cat# V4XC-2012 KT Streptavidin microbeads Miltenyi Biotec Cat# 130-048-101 LD columns Miltenyi Biotec Cat# 130-042-901 (Continued on next page) Immunity 59, 270–287.e1–e11, February 10, 2026 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Combined snRNA-Seq and snATAC-Seq primary splenic pDCs Pucella et al. 72 GEO: GSE252189 Experimental models: Cell lines Mouse: L929 ATCC Cat# CCL-1, RRID:CVCL_0462 Mouse: B16-Flt3L Mach et al. 82 N/A Mouse: HoxB8-FL Redecke et al. 83 N/A Mouse: Pmi28 c/o C. Cardoso N/A African green monkey: Vero E6 ATCC Cat# CRL1586; RRID: CVCL_0574 Experimental models: Organisms/strains Mouse: Smc3 fl : B6(SJL)Smc3 tm1c(EUCOMM)Wtsi /J The Jackson Laboratory RRID: IMSR_JAX:030559 Mouse: Rosa26 CreERT2 Dr. Thomas Ludwig, Ohio State University N/A Mouse: Ifn ΔTB2 This paper N/A Mouse: Ifn ΔTB2/TB4 This paper N/A Mouse: Ifn ΔTB2/TB3/TB4 This paper N/A Mouse: Irf8 -/- : B6(Cg)-Irf8 tm1.2Hm /J The Jackson Laboratory RRID:IMSR_JAX:018298 Mouse: Ctrl for snRNA-Seq/snATAC-Seq: C57BL/6-Tg(CAG-EGFP)131Osb/LeySopJ The Jackson Laboratory RRID:IMSR_JAX:006567 Mouse: C57BL/6: C57BL/6NTac Taconic Biosciences Model# B6-F Mouse: BALB/c: BALB/cAnNTac Taconic Biosciences Model# BALB-M Oligonucleotides sgRNAs targeting IFN-I TB in mouse germline, see Table S6 This paper N/A sgRNAs for targeting in HoxB8-FL cells, see Table S6 This paper N/A Primers for genotyping of Ifn ΔTB2 , Ifn ΔTB3 , and Ifn ΔTB4 alleles, see Table S6 This paper N/A RT-qPCR primers for Ifn subtype quantification, see Table S6 This paper N/A Alt-R CRISPR-Cas9 Negative Control crRNA #1 IDT Cat# 1072544 Alt-R CRISPR-Cas9 tracrRNA IDT Cat# 1072532 BAC clones RP23-64G11, RP23-139P14 and RP23-360M23 BACPAC Resources N/A Software and algorithms FlowJo v10 Tree Star RRID: SCR_008520 GraphPad Prism 10 Graphpad Inc RRID: SCR_002798 ExAtlas https://kolab.elixirgensci.com/exatlas/ N/A Heatmapper http://www.heatmapper.ca/expression/ RRID:SCR_016974 IGV https://igv.org/ RRID:SCR_011793 Resgen https://resgen.io/ N/A ImageJ https://imagej.net/ij/ RRID:SCR_003070 bcl2fastq Illumina RRID:SCR_015058 Seq-N-Slide https://igordot.github.io/sns/ RRID:SCR_021752 Trimmomatic http://www.usadellab.org/cms/ ?page=trimmomatic RRID:SCR_011848 STAR aligner https://github.com/alexdobin/STAR RRID:SCR_004463 featureCounts https://subread.sourceforge.net/ featureCounts.html RRID:SCR_012919 DESeq2 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html RRID:SCR_015687 (Continued on next page) Immunity 59, 270–287.e1–e11, February 10, 2026 e4
High Molecular Weight:Article Title: Chromatin-mediated anticipatory control of type I interferon production in plasmacytoid dendritic cells.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Goat anti-lamin B (M-20) Santa Cruz Biotechnology Cat# sc-6217; RRID:AB_648158 Rabbit anti-SMC3 mAb (D47B5) Cell Signaling Technology Cat# 5696; RRID:AB_10705575 AlexaFluor 488 goat anti-rabbit IgG (H+L) Thermo Fisher Scientific Cat# A-11034; RRID:AB_2576217 Rabbit anti-β-actin (RM112) Thermo Fisher Scientific Cat# MA5-33078; RRID:AB_2810170 Bacterial and virus strains SARS-CoV-2 MA10 BEI Resources NR-55329 Sendai virus (Cantell strain) Charles River (c/o D. Levy) N/A Chemicals, peptides, and recombinant proteins β-Estradiol Sigma-Aldrich Cat# E2758 Alt-R S.p. .. Cas9 Nuclease V3 IDT Cat# 1081058 Alt-R Cas9 Electroporation Enhancer IDT Cat# 1075915 (Z)-4-Hydroxytamoxifen Sigma-Aldrich Cat# H7904 Recombinant Mouse GM-CSF (carrier free) BioLegend Cat# 576304 Recombinant Mouse IL-4 (carrier free) BioLegend Cat# 574304 Collagenase D from Clostridium histolyticum Roche Cat# 11088858001 Pronase from Streptomyces griseus Millipore Sigma Cat# 10165921001 Amphotericin B solution Millipore Sigma Cat# A2942 Liberase TL Roche Cat# 5401020001 Bovine Serum Albumin, Powder Millipore Sigma Cat# A9647 Deoxyribonuclease I (DNase I) from bovine pancreas Roche Cat# 11284932001 RBC Lysis Buffer BioLegend Cat# 420301 Poly(I:C) high molecular weight InvivoGen Cat# tlrl-pic CpG-A/ODN 2216 InvivoGen Cat# tlrl-2216-1 DOTAP chloride MedChemExpress Cat# HY-112754A Concanavalin A from Canavalia ensiformis Millipore Sigma Cat# L7647, C2010 Paraformaldehyde Aqueous Solution, EM Grade Electron Microscopy Sciences Cat# 15710 Triton X-100 Thermo Fisher Scientific Cat# 85111 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific Cat# 89900 Halt Protease Inhibitor Cocktail Thermo Fisher Scientific Cat# 78429 Halt Phosphatase Inhibitor Cocktail Thermo Fisher Scientific Cat# 78420 NuPAGE LDS Sample Buffer Thermo Fisher Scientific Cat# NP0007 NuPAGE Sample Reducing Agent Thermo Fisher Scientific Cat# NP0009 TRIzol Reagent Themo Fisher Scientific Cat# 15596018 Digitonin Thermo Fisher Scientific Cat# BN2006 Trizma Hydrochloride Solution, pH 7.4 Millipore Sigma Cat# T2194 Sodium Chloride Solution, 5 M Millipore Sigma Cat# 59222C Magnesium Chloride Solution, 1M Millipore Sigma Cat# M1028 IGEPAL CA-630 Millipore Sigma Cat# I8896 Protector RNase inhibitor Millipore Sigma Cat# 3335402001 DL-Dithiothreitol (DTT) solution, 1M Millipore Sigma Cat# 646563 Bovine Serum Albumin Fraction V Solution Thermo Fisher Scientific Cat# 15260037 10% Tween 20 Bio-Rad Cat# 1662404 Critical commercial assays Venor GeM Mycoplasma PCR-Based Detection Kit Sigma-Aldrich Cat# MP0025 SF Cell Line 4D-Nucleofector X Kit L Lonza Cat# V4XC-2012 KT Streptavidin microbeads Miltenyi Biotec Cat# 130-048-101 LD columns Miltenyi Biotec Cat# 130-042-901 (Continued on next page) Immunity 59, 270–287.e1–e11, February 10, 2026 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Combined snRNA-Seq and snATAC-Seq primary splenic pDCs Pucella et al. 72 GEO: GSE252189 Experimental models: Cell lines Mouse: L929 ATCC Cat# CCL-1, RRID:CVCL_0462 Mouse: B16-Flt3L Mach et al. 82 N/A Mouse: HoxB8-FL Redecke et al. 83 N/A Mouse: Pmi28 c/o C. Cardoso N/A African green monkey: Vero E6 ATCC Cat# CRL1586; RRID: CVCL_0574 Experimental models: Organisms/strains Mouse: Smc3 fl : B6(SJL)Smc3 tm1c(EUCOMM)Wtsi /J The Jackson Laboratory RRID: IMSR_JAX:030559 Mouse: Rosa26 CreERT2 Dr. Thomas Ludwig, Ohio State University N/A Mouse: Ifn ΔTB2 This paper N/A Mouse: Ifn ΔTB2/TB4 This paper N/A Mouse: Ifn ΔTB2/TB3/TB4 This paper N/A Mouse: Irf8 -/- : B6(Cg)-Irf8 tm1.2Hm /J The Jackson Laboratory RRID:IMSR_JAX:018298 Mouse: Ctrl for snRNA-Seq/snATAC-Seq: C57BL/6-Tg(CAG-EGFP)131Osb/LeySopJ The Jackson Laboratory RRID:IMSR_JAX:006567 Mouse: C57BL/6: C57BL/6NTac Taconic Biosciences Model# B6-F Mouse: BALB/c: BALB/cAnNTac Taconic Biosciences Model# BALB-M Oligonucleotides sgRNAs targeting IFN-I TB in mouse germline, see Table S6 This paper N/A sgRNAs for targeting in HoxB8-FL cells, see Table S6 This paper N/A Primers for genotyping of Ifn ΔTB2 , Ifn ΔTB3 , and Ifn ΔTB4 alleles, see Table S6 This paper N/A RT-qPCR primers for Ifn subtype quantification, see Table S6 This paper N/A Alt-R CRISPR-Cas9 Negative Control crRNA #1 IDT Cat# 1072544 Alt-R CRISPR-Cas9 tracrRNA IDT Cat# 1072532 BAC clones RP23-64G11, RP23-139P14 and RP23-360M23 BACPAC Resources N/A Software and algorithms FlowJo v10 Tree Star RRID: SCR_008520 GraphPad Prism 10 Graphpad Inc RRID: SCR_002798 ExAtlas https://kolab.elixirgensci.com/exatlas/ N/A Heatmapper http://www.heatmapper.ca/expression/ RRID:SCR_016974 IGV https://igv.org/ RRID:SCR_011793 Resgen https://resgen.io/ N/A ImageJ https://imagej.net/ij/ RRID:SCR_003070 bcl2fastq Illumina RRID:SCR_015058 Seq-N-Slide https://igordot.github.io/sns/ RRID:SCR_021752 Trimmomatic http://www.usadellab.org/cms/ ?page=trimmomatic RRID:SCR_011848 STAR aligner https://github.com/alexdobin/STAR RRID:SCR_004463 featureCounts https://subread.sourceforge.net/ featureCounts.html RRID:SCR_012919 DESeq2 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html RRID:SCR_015687 (Continued on next page) Immunity 59, 270–287.e1–e11, February 10, 2026 e4
Electron Microscopy:Article Title: Chromatin-mediated anticipatory control of type I interferon production in plasmacytoid dendritic cells.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Goat anti-lamin B (M-20) Santa Cruz Biotechnology Cat# sc-6217; RRID:AB_648158 Rabbit anti-SMC3 mAb (D47B5) Cell Signaling Technology Cat# 5696; RRID:AB_10705575 AlexaFluor 488 goat anti-rabbit IgG (H+L) Thermo Fisher Scientific Cat# A-11034; RRID:AB_2576217 Rabbit anti-β-actin (RM112) Thermo Fisher Scientific Cat# MA5-33078; RRID:AB_2810170 Bacterial and virus strains SARS-CoV-2 MA10 BEI Resources NR-55329 Sendai virus (Cantell strain) Charles River (c/o D. Levy) N/A Chemicals, peptides, and recombinant proteins β-Estradiol Sigma-Aldrich Cat# E2758 Alt-R S.p. .. Cas9 Nuclease V3 IDT Cat# 1081058 Alt-R Cas9 Electroporation Enhancer IDT Cat# 1075915 (Z)-4-Hydroxytamoxifen Sigma-Aldrich Cat# H7904 Recombinant Mouse GM-CSF (carrier free) BioLegend Cat# 576304 Recombinant Mouse IL-4 (carrier free) BioLegend Cat# 574304 Collagenase D from Clostridium histolyticum Roche Cat# 11088858001 Pronase from Streptomyces griseus Millipore Sigma Cat# 10165921001 Amphotericin B solution Millipore Sigma Cat# A2942 Liberase TL Roche Cat# 5401020001 Bovine Serum Albumin, Powder Millipore Sigma Cat# A9647 Deoxyribonuclease I (DNase I) from bovine pancreas Roche Cat# 11284932001 RBC Lysis Buffer BioLegend Cat# 420301 Poly(I:C) high molecular weight InvivoGen Cat# tlrl-pic CpG-A/ODN 2216 InvivoGen Cat# tlrl-2216-1 DOTAP chloride MedChemExpress Cat# HY-112754A Concanavalin A from Canavalia ensiformis Millipore Sigma Cat# L7647, C2010 Paraformaldehyde Aqueous Solution, EM Grade Electron Microscopy Sciences Cat# 15710 Triton X-100 Thermo Fisher Scientific Cat# 85111 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific Cat# 89900 Halt Protease Inhibitor Cocktail Thermo Fisher Scientific Cat# 78429 Halt Phosphatase Inhibitor Cocktail Thermo Fisher Scientific Cat# 78420 NuPAGE LDS Sample Buffer Thermo Fisher Scientific Cat# NP0007 NuPAGE Sample Reducing Agent Thermo Fisher Scientific Cat# NP0009 TRIzol Reagent Themo Fisher Scientific Cat# 15596018 Digitonin Thermo Fisher Scientific Cat# BN2006 Trizma Hydrochloride Solution, pH 7.4 Millipore Sigma Cat# T2194 Sodium Chloride Solution, 5 M Millipore Sigma Cat# 59222C Magnesium Chloride Solution, 1M Millipore Sigma Cat# M1028 IGEPAL CA-630 Millipore Sigma Cat# I8896 Protector RNase inhibitor Millipore Sigma Cat# 3335402001 DL-Dithiothreitol (DTT) solution, 1M Millipore Sigma Cat# 646563 Bovine Serum Albumin Fraction V Solution Thermo Fisher Scientific Cat# 15260037 10% Tween 20 Bio-Rad Cat# 1662404 Critical commercial assays Venor GeM Mycoplasma PCR-Based Detection Kit Sigma-Aldrich Cat# MP0025 SF Cell Line 4D-Nucleofector X Kit L Lonza Cat# V4XC-2012 KT Streptavidin microbeads Miltenyi Biotec Cat# 130-048-101 LD columns Miltenyi Biotec Cat# 130-042-901 (Continued on next page) Immunity 59, 270–287.e1–e11, February 10, 2026 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Combined snRNA-Seq and snATAC-Seq primary splenic pDCs Pucella et al. 72 GEO: GSE252189 Experimental models: Cell lines Mouse: L929 ATCC Cat# CCL-1, RRID:CVCL_0462 Mouse: B16-Flt3L Mach et al. 82 N/A Mouse: HoxB8-FL Redecke et al. 83 N/A Mouse: Pmi28 c/o C. Cardoso N/A African green monkey: Vero E6 ATCC Cat# CRL1586; RRID: CVCL_0574 Experimental models: Organisms/strains Mouse: Smc3 fl : B6(SJL)Smc3 tm1c(EUCOMM)Wtsi /J The Jackson Laboratory RRID: IMSR_JAX:030559 Mouse: Rosa26 CreERT2 Dr. Thomas Ludwig, Ohio State University N/A Mouse: Ifn ΔTB2 This paper N/A Mouse: Ifn ΔTB2/TB4 This paper N/A Mouse: Ifn ΔTB2/TB3/TB4 This paper N/A Mouse: Irf8 -/- : B6(Cg)-Irf8 tm1.2Hm /J The Jackson Laboratory RRID:IMSR_JAX:018298 Mouse: Ctrl for snRNA-Seq/snATAC-Seq: C57BL/6-Tg(CAG-EGFP)131Osb/LeySopJ The Jackson Laboratory RRID:IMSR_JAX:006567 Mouse: C57BL/6: C57BL/6NTac Taconic Biosciences Model# B6-F Mouse: BALB/c: BALB/cAnNTac Taconic Biosciences Model# BALB-M Oligonucleotides sgRNAs targeting IFN-I TB in mouse germline, see Table S6 This paper N/A sgRNAs for targeting in HoxB8-FL cells, see Table S6 This paper N/A Primers for genotyping of Ifn ΔTB2 , Ifn ΔTB3 , and Ifn ΔTB4 alleles, see Table S6 This paper N/A RT-qPCR primers for Ifn subtype quantification, see Table S6 This paper N/A Alt-R CRISPR-Cas9 Negative Control crRNA #1 IDT Cat# 1072544 Alt-R CRISPR-Cas9 tracrRNA IDT Cat# 1072532 BAC clones RP23-64G11, RP23-139P14 and RP23-360M23 BACPAC Resources N/A Software and algorithms FlowJo v10 Tree Star RRID: SCR_008520 GraphPad Prism 10 Graphpad Inc RRID: SCR_002798 ExAtlas https://kolab.elixirgensci.com/exatlas/ N/A Heatmapper http://www.heatmapper.ca/expression/ RRID:SCR_016974 IGV https://igv.org/ RRID:SCR_011793 Resgen https://resgen.io/ N/A ImageJ https://imagej.net/ij/ RRID:SCR_003070 bcl2fastq Illumina RRID:SCR_015058 Seq-N-Slide https://igordot.github.io/sns/ RRID:SCR_021752 Trimmomatic http://www.usadellab.org/cms/ ?page=trimmomatic RRID:SCR_011848 STAR aligner https://github.com/alexdobin/STAR RRID:SCR_004463 featureCounts https://subread.sourceforge.net/ featureCounts.html RRID:SCR_012919 DESeq2 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html RRID:SCR_015687 (Continued on next page) Immunity 59, 270–287.e1–e11, February 10, 2026 e4
Lysis:Article Title: Chromatin-mediated anticipatory control of type I interferon production in plasmacytoid dendritic cells.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Goat anti-lamin B (M-20) Santa Cruz Biotechnology Cat# sc-6217; RRID:AB_648158 Rabbit anti-SMC3 mAb (D47B5) Cell Signaling Technology Cat# 5696; RRID:AB_10705575 AlexaFluor 488 goat anti-rabbit IgG (H+L) Thermo Fisher Scientific Cat# A-11034; RRID:AB_2576217 Rabbit anti-β-actin (RM112) Thermo Fisher Scientific Cat# MA5-33078; RRID:AB_2810170 Bacterial and virus strains SARS-CoV-2 MA10 BEI Resources NR-55329 Sendai virus (Cantell strain) Charles River (c/o D. Levy) N/A Chemicals, peptides, and recombinant proteins β-Estradiol Sigma-Aldrich Cat# E2758 Alt-R S.p. .. Cas9 Nuclease V3 IDT Cat# 1081058 Alt-R Cas9 Electroporation Enhancer IDT Cat# 1075915 (Z)-4-Hydroxytamoxifen Sigma-Aldrich Cat# H7904 Recombinant Mouse GM-CSF (carrier free) BioLegend Cat# 576304 Recombinant Mouse IL-4 (carrier free) BioLegend Cat# 574304 Collagenase D from Clostridium histolyticum Roche Cat# 11088858001 Pronase from Streptomyces griseus Millipore Sigma Cat# 10165921001 Amphotericin B solution Millipore Sigma Cat# A2942 Liberase TL Roche Cat# 5401020001 Bovine Serum Albumin, Powder Millipore Sigma Cat# A9647 Deoxyribonuclease I (DNase I) from bovine pancreas Roche Cat# 11284932001 RBC Lysis Buffer BioLegend Cat# 420301 Poly(I:C) high molecular weight InvivoGen Cat# tlrl-pic CpG-A/ODN 2216 InvivoGen Cat# tlrl-2216-1 DOTAP chloride MedChemExpress Cat# HY-112754A Concanavalin A from Canavalia ensiformis Millipore Sigma Cat# L7647, C2010 Paraformaldehyde Aqueous Solution, EM Grade Electron Microscopy Sciences Cat# 15710 Triton X-100 Thermo Fisher Scientific Cat# 85111 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific Cat# 89900 Halt Protease Inhibitor Cocktail Thermo Fisher Scientific Cat# 78429 Halt Phosphatase Inhibitor Cocktail Thermo Fisher Scientific Cat# 78420 NuPAGE LDS Sample Buffer Thermo Fisher Scientific Cat# NP0007 NuPAGE Sample Reducing Agent Thermo Fisher Scientific Cat# NP0009 TRIzol Reagent Themo Fisher Scientific Cat# 15596018 Digitonin Thermo Fisher Scientific Cat# BN2006 Trizma Hydrochloride Solution, pH 7.4 Millipore Sigma Cat# T2194 Sodium Chloride Solution, 5 M Millipore Sigma Cat# 59222C Magnesium Chloride Solution, 1M Millipore Sigma Cat# M1028 IGEPAL CA-630 Millipore Sigma Cat# I8896 Protector RNase inhibitor Millipore Sigma Cat# 3335402001 DL-Dithiothreitol (DTT) solution, 1M Millipore Sigma Cat# 646563 Bovine Serum Albumin Fraction V Solution Thermo Fisher Scientific Cat# 15260037 10% Tween 20 Bio-Rad Cat# 1662404 Critical commercial assays Venor GeM Mycoplasma PCR-Based Detection Kit Sigma-Aldrich Cat# MP0025 SF Cell Line 4D-Nucleofector X Kit L Lonza Cat# V4XC-2012 KT Streptavidin microbeads Miltenyi Biotec Cat# 130-048-101 LD columns Miltenyi Biotec Cat# 130-042-901 (Continued on next page) Immunity 59, 270–287.e1–e11, February 10, 2026 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Combined snRNA-Seq and snATAC-Seq primary splenic pDCs Pucella et al. 72 GEO: GSE252189 Experimental models: Cell lines Mouse: L929 ATCC Cat# CCL-1, RRID:CVCL_0462 Mouse: B16-Flt3L Mach et al. 82 N/A Mouse: HoxB8-FL Redecke et al. 83 N/A Mouse: Pmi28 c/o C. Cardoso N/A African green monkey: Vero E6 ATCC Cat# CRL1586; RRID: CVCL_0574 Experimental models: Organisms/strains Mouse: Smc3 fl : B6(SJL)Smc3 tm1c(EUCOMM)Wtsi /J The Jackson Laboratory RRID: IMSR_JAX:030559 Mouse: Rosa26 CreERT2 Dr. Thomas Ludwig, Ohio State University N/A Mouse: Ifn ΔTB2 This paper N/A Mouse: Ifn ΔTB2/TB4 This paper N/A Mouse: Ifn ΔTB2/TB3/TB4 This paper N/A Mouse: Irf8 -/- : B6(Cg)-Irf8 tm1.2Hm /J The Jackson Laboratory RRID:IMSR_JAX:018298 Mouse: Ctrl for snRNA-Seq/snATAC-Seq: C57BL/6-Tg(CAG-EGFP)131Osb/LeySopJ The Jackson Laboratory RRID:IMSR_JAX:006567 Mouse: C57BL/6: C57BL/6NTac Taconic Biosciences Model# B6-F Mouse: BALB/c: BALB/cAnNTac Taconic Biosciences Model# BALB-M Oligonucleotides sgRNAs targeting IFN-I TB in mouse germline, see Table S6 This paper N/A sgRNAs for targeting in HoxB8-FL cells, see Table S6 This paper N/A Primers for genotyping of Ifn ΔTB2 , Ifn ΔTB3 , and Ifn ΔTB4 alleles, see Table S6 This paper N/A RT-qPCR primers for Ifn subtype quantification, see Table S6 This paper N/A Alt-R CRISPR-Cas9 Negative Control crRNA #1 IDT Cat# 1072544 Alt-R CRISPR-Cas9 tracrRNA IDT Cat# 1072532 BAC clones RP23-64G11, RP23-139P14 and RP23-360M23 BACPAC Resources N/A Software and algorithms FlowJo v10 Tree Star RRID: SCR_008520 GraphPad Prism 10 Graphpad Inc RRID: SCR_002798 ExAtlas https://kolab.elixirgensci.com/exatlas/ N/A Heatmapper http://www.heatmapper.ca/expression/ RRID:SCR_016974 IGV https://igv.org/ RRID:SCR_011793 Resgen https://resgen.io/ N/A ImageJ https://imagej.net/ij/ RRID:SCR_003070 bcl2fastq Illumina RRID:SCR_015058 Seq-N-Slide https://igordot.github.io/sns/ RRID:SCR_021752 Trimmomatic http://www.usadellab.org/cms/ ?page=trimmomatic RRID:SCR_011848 STAR aligner https://github.com/alexdobin/STAR RRID:SCR_004463 featureCounts https://subread.sourceforge.net/ featureCounts.html RRID:SCR_012919 DESeq2 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html RRID:SCR_015687 (Continued on next page) Immunity 59, 270–287.e1–e11, February 10, 2026 e4
Extraction:Article Title: Chromatin-mediated anticipatory control of type I interferon production in plasmacytoid dendritic cells.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Goat anti-lamin B (M-20) Santa Cruz Biotechnology Cat# sc-6217; RRID:AB_648158 Rabbit anti-SMC3 mAb (D47B5) Cell Signaling Technology Cat# 5696; RRID:AB_10705575 AlexaFluor 488 goat anti-rabbit IgG (H+L) Thermo Fisher Scientific Cat# A-11034; RRID:AB_2576217 Rabbit anti-β-actin (RM112) Thermo Fisher Scientific Cat# MA5-33078; RRID:AB_2810170 Bacterial and virus strains SARS-CoV-2 MA10 BEI Resources NR-55329 Sendai virus (Cantell strain) Charles River (c/o D. Levy) N/A Chemicals, peptides, and recombinant proteins β-Estradiol Sigma-Aldrich Cat# E2758 Alt-R S.p. .. Cas9 Nuclease V3 IDT Cat# 1081058 Alt-R Cas9 Electroporation Enhancer IDT Cat# 1075915 (Z)-4-Hydroxytamoxifen Sigma-Aldrich Cat# H7904 Recombinant Mouse GM-CSF (carrier free) BioLegend Cat# 576304 Recombinant Mouse IL-4 (carrier free) BioLegend Cat# 574304 Collagenase D from Clostridium histolyticum Roche Cat# 11088858001 Pronase from Streptomyces griseus Millipore Sigma Cat# 10165921001 Amphotericin B solution Millipore Sigma Cat# A2942 Liberase TL Roche Cat# 5401020001 Bovine Serum Albumin, Powder Millipore Sigma Cat# A9647 Deoxyribonuclease I (DNase I) from bovine pancreas Roche Cat# 11284932001 RBC Lysis Buffer BioLegend Cat# 420301 Poly(I:C) high molecular weight InvivoGen Cat# tlrl-pic CpG-A/ODN 2216 InvivoGen Cat# tlrl-2216-1 DOTAP chloride MedChemExpress Cat# HY-112754A Concanavalin A from Canavalia ensiformis Millipore Sigma Cat# L7647, C2010 Paraformaldehyde Aqueous Solution, EM Grade Electron Microscopy Sciences Cat# 15710 Triton X-100 Thermo Fisher Scientific Cat# 85111 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific Cat# 89900 Halt Protease Inhibitor Cocktail Thermo Fisher Scientific Cat# 78429 Halt Phosphatase Inhibitor Cocktail Thermo Fisher Scientific Cat# 78420 NuPAGE LDS Sample Buffer Thermo Fisher Scientific Cat# NP0007 NuPAGE Sample Reducing Agent Thermo Fisher Scientific Cat# NP0009 TRIzol Reagent Themo Fisher Scientific Cat# 15596018 Digitonin Thermo Fisher Scientific Cat# BN2006 Trizma Hydrochloride Solution, pH 7.4 Millipore Sigma Cat# T2194 Sodium Chloride Solution, 5 M Millipore Sigma Cat# 59222C Magnesium Chloride Solution, 1M Millipore Sigma Cat# M1028 IGEPAL CA-630 Millipore Sigma Cat# I8896 Protector RNase inhibitor Millipore Sigma Cat# 3335402001 DL-Dithiothreitol (DTT) solution, 1M Millipore Sigma Cat# 646563 Bovine Serum Albumin Fraction V Solution Thermo Fisher Scientific Cat# 15260037 10% Tween 20 Bio-Rad Cat# 1662404 Critical commercial assays Venor GeM Mycoplasma PCR-Based Detection Kit Sigma-Aldrich Cat# MP0025 SF Cell Line 4D-Nucleofector X Kit L Lonza Cat# V4XC-2012 KT Streptavidin microbeads Miltenyi Biotec Cat# 130-048-101 LD columns Miltenyi Biotec Cat# 130-042-901 (Continued on next page) Immunity 59, 270–287.e1–e11, February 10, 2026 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Combined snRNA-Seq and snATAC-Seq primary splenic pDCs Pucella et al. 72 GEO: GSE252189 Experimental models: Cell lines Mouse: L929 ATCC Cat# CCL-1, RRID:CVCL_0462 Mouse: B16-Flt3L Mach et al. 82 N/A Mouse: HoxB8-FL Redecke et al. 83 N/A Mouse: Pmi28 c/o C. Cardoso N/A African green monkey: Vero E6 ATCC Cat# CRL1586; RRID: CVCL_0574 Experimental models: Organisms/strains Mouse: Smc3 fl : B6(SJL)Smc3 tm1c(EUCOMM)Wtsi /J The Jackson Laboratory RRID: IMSR_JAX:030559 Mouse: Rosa26 CreERT2 Dr. Thomas Ludwig, Ohio State University N/A Mouse: Ifn ΔTB2 This paper N/A Mouse: Ifn ΔTB2/TB4 This paper N/A Mouse: Ifn ΔTB2/TB3/TB4 This paper N/A Mouse: Irf8 -/- : B6(Cg)-Irf8 tm1.2Hm /J The Jackson Laboratory RRID:IMSR_JAX:018298 Mouse: Ctrl for snRNA-Seq/snATAC-Seq: C57BL/6-Tg(CAG-EGFP)131Osb/LeySopJ The Jackson Laboratory RRID:IMSR_JAX:006567 Mouse: C57BL/6: C57BL/6NTac Taconic Biosciences Model# B6-F Mouse: BALB/c: BALB/cAnNTac Taconic Biosciences Model# BALB-M Oligonucleotides sgRNAs targeting IFN-I TB in mouse germline, see Table S6 This paper N/A sgRNAs for targeting in HoxB8-FL cells, see Table S6 This paper N/A Primers for genotyping of Ifn ΔTB2 , Ifn ΔTB3 , and Ifn ΔTB4 alleles, see Table S6 This paper N/A RT-qPCR primers for Ifn subtype quantification, see Table S6 This paper N/A Alt-R CRISPR-Cas9 Negative Control crRNA #1 IDT Cat# 1072544 Alt-R CRISPR-Cas9 tracrRNA IDT Cat# 1072532 BAC clones RP23-64G11, RP23-139P14 and RP23-360M23 BACPAC Resources N/A Software and algorithms FlowJo v10 Tree Star RRID: SCR_008520 GraphPad Prism 10 Graphpad Inc RRID: SCR_002798 ExAtlas https://kolab.elixirgensci.com/exatlas/ N/A Heatmapper http://www.heatmapper.ca/expression/ RRID:SCR_016974 IGV https://igv.org/ RRID:SCR_011793 Resgen https://resgen.io/ N/A ImageJ https://imagej.net/ij/ RRID:SCR_003070 bcl2fastq Illumina RRID:SCR_015058 Seq-N-Slide https://igordot.github.io/sns/ RRID:SCR_021752 Trimmomatic http://www.usadellab.org/cms/ ?page=trimmomatic RRID:SCR_011848 STAR aligner https://github.com/alexdobin/STAR RRID:SCR_004463 featureCounts https://subread.sourceforge.net/ featureCounts.html RRID:SCR_012919 DESeq2 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html RRID:SCR_015687 (Continued on next page) Immunity 59, 270–287.e1–e11, February 10, 2026 e4
Protease Inhibitor:Article Title: Chromatin-mediated anticipatory control of type I interferon production in plasmacytoid dendritic cells.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Goat anti-lamin B (M-20) Santa Cruz Biotechnology Cat# sc-6217; RRID:AB_648158 Rabbit anti-SMC3 mAb (D47B5) Cell Signaling Technology Cat# 5696; RRID:AB_10705575 AlexaFluor 488 goat anti-rabbit IgG (H+L) Thermo Fisher Scientific Cat# A-11034; RRID:AB_2576217 Rabbit anti-β-actin (RM112) Thermo Fisher Scientific Cat# MA5-33078; RRID:AB_2810170 Bacterial and virus strains SARS-CoV-2 MA10 BEI Resources NR-55329 Sendai virus (Cantell strain) Charles River (c/o D. Levy) N/A Chemicals, peptides, and recombinant proteins β-Estradiol Sigma-Aldrich Cat# E2758 Alt-R S.p. .. Cas9 Nuclease V3 IDT Cat# 1081058 Alt-R Cas9 Electroporation Enhancer IDT Cat# 1075915 (Z)-4-Hydroxytamoxifen Sigma-Aldrich Cat# H7904 Recombinant Mouse GM-CSF (carrier free) BioLegend Cat# 576304 Recombinant Mouse IL-4 (carrier free) BioLegend Cat# 574304 Collagenase D from Clostridium histolyticum Roche Cat# 11088858001 Pronase from Streptomyces griseus Millipore Sigma Cat# 10165921001 Amphotericin B solution Millipore Sigma Cat# A2942 Liberase TL Roche Cat# 5401020001 Bovine Serum Albumin, Powder Millipore Sigma Cat# A9647 Deoxyribonuclease I (DNase I) from bovine pancreas Roche Cat# 11284932001 RBC Lysis Buffer BioLegend Cat# 420301 Poly(I:C) high molecular weight InvivoGen Cat# tlrl-pic CpG-A/ODN 2216 InvivoGen Cat# tlrl-2216-1 DOTAP chloride MedChemExpress Cat# HY-112754A Concanavalin A from Canavalia ensiformis Millipore Sigma Cat# L7647, C2010 Paraformaldehyde Aqueous Solution, EM Grade Electron Microscopy Sciences Cat# 15710 Triton X-100 Thermo Fisher Scientific Cat# 85111 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific Cat# 89900 Halt Protease Inhibitor Cocktail Thermo Fisher Scientific Cat# 78429 Halt Phosphatase Inhibitor Cocktail Thermo Fisher Scientific Cat# 78420 NuPAGE LDS Sample Buffer Thermo Fisher Scientific Cat# NP0007 NuPAGE Sample Reducing Agent Thermo Fisher Scientific Cat# NP0009 TRIzol Reagent Themo Fisher Scientific Cat# 15596018 Digitonin Thermo Fisher Scientific Cat# BN2006 Trizma Hydrochloride Solution, pH 7.4 Millipore Sigma Cat# T2194 Sodium Chloride Solution, 5 M Millipore Sigma Cat# 59222C Magnesium Chloride Solution, 1M Millipore Sigma Cat# M1028 IGEPAL CA-630 Millipore Sigma Cat# I8896 Protector RNase inhibitor Millipore Sigma Cat# 3335402001 DL-Dithiothreitol (DTT) solution, 1M Millipore Sigma Cat# 646563 Bovine Serum Albumin Fraction V Solution Thermo Fisher Scientific Cat# 15260037 10% Tween 20 Bio-Rad Cat# 1662404 Critical commercial assays Venor GeM Mycoplasma PCR-Based Detection Kit Sigma-Aldrich Cat# MP0025 SF Cell Line 4D-Nucleofector X Kit L Lonza Cat# V4XC-2012 KT Streptavidin microbeads Miltenyi Biotec Cat# 130-048-101 LD columns Miltenyi Biotec Cat# 130-042-901 (Continued on next page) Immunity 59, 270–287.e1–e11, February 10, 2026 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Combined snRNA-Seq and snATAC-Seq primary splenic pDCs Pucella et al. 72 GEO: GSE252189 Experimental models: Cell lines Mouse: L929 ATCC Cat# CCL-1, RRID:CVCL_0462 Mouse: B16-Flt3L Mach et al. 82 N/A Mouse: HoxB8-FL Redecke et al. 83 N/A Mouse: Pmi28 c/o C. Cardoso N/A African green monkey: Vero E6 ATCC Cat# CRL1586; RRID: CVCL_0574 Experimental models: Organisms/strains Mouse: Smc3 fl : B6(SJL)Smc3 tm1c(EUCOMM)Wtsi /J The Jackson Laboratory RRID: IMSR_JAX:030559 Mouse: Rosa26 CreERT2 Dr. Thomas Ludwig, Ohio State University N/A Mouse: Ifn ΔTB2 This paper N/A Mouse: Ifn ΔTB2/TB4 This paper N/A Mouse: Ifn ΔTB2/TB3/TB4 This paper N/A Mouse: Irf8 -/- : B6(Cg)-Irf8 tm1.2Hm /J The Jackson Laboratory RRID:IMSR_JAX:018298 Mouse: Ctrl for snRNA-Seq/snATAC-Seq: C57BL/6-Tg(CAG-EGFP)131Osb/LeySopJ The Jackson Laboratory RRID:IMSR_JAX:006567 Mouse: C57BL/6: C57BL/6NTac Taconic Biosciences Model# B6-F Mouse: BALB/c: BALB/cAnNTac Taconic Biosciences Model# BALB-M Oligonucleotides sgRNAs targeting IFN-I TB in mouse germline, see Table S6 This paper N/A sgRNAs for targeting in HoxB8-FL cells, see Table S6 This paper N/A Primers for genotyping of Ifn ΔTB2 , Ifn ΔTB3 , and Ifn ΔTB4 alleles, see Table S6 This paper N/A RT-qPCR primers for Ifn subtype quantification, see Table S6 This paper N/A Alt-R CRISPR-Cas9 Negative Control crRNA #1 IDT Cat# 1072544 Alt-R CRISPR-Cas9 tracrRNA IDT Cat# 1072532 BAC clones RP23-64G11, RP23-139P14 and RP23-360M23 BACPAC Resources N/A Software and algorithms FlowJo v10 Tree Star RRID: SCR_008520 GraphPad Prism 10 Graphpad Inc RRID: SCR_002798 ExAtlas https://kolab.elixirgensci.com/exatlas/ N/A Heatmapper http://www.heatmapper.ca/expression/ RRID:SCR_016974 IGV https://igv.org/ RRID:SCR_011793 Resgen https://resgen.io/ N/A ImageJ https://imagej.net/ij/ RRID:SCR_003070 bcl2fastq Illumina RRID:SCR_015058 Seq-N-Slide https://igordot.github.io/sns/ RRID:SCR_021752 Trimmomatic http://www.usadellab.org/cms/ ?page=trimmomatic RRID:SCR_011848 STAR aligner https://github.com/alexdobin/STAR RRID:SCR_004463 featureCounts https://subread.sourceforge.net/ featureCounts.html RRID:SCR_012919 DESeq2 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html RRID:SCR_015687 (Continued on next page) Immunity 59, 270–287.e1–e11, February 10, 2026 e4
Polymerase Chain Reaction:Article Title: Chromatin-mediated anticipatory control of type I interferon production in plasmacytoid dendritic cells.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Goat anti-lamin B (M-20) Santa Cruz Biotechnology Cat# sc-6217; RRID:AB_648158 Rabbit anti-SMC3 mAb (D47B5) Cell Signaling Technology Cat# 5696; RRID:AB_10705575 AlexaFluor 488 goat anti-rabbit IgG (H+L) Thermo Fisher Scientific Cat# A-11034; RRID:AB_2576217 Rabbit anti-β-actin (RM112) Thermo Fisher Scientific Cat# MA5-33078; RRID:AB_2810170 Bacterial and virus strains SARS-CoV-2 MA10 BEI Resources NR-55329 Sendai virus (Cantell strain) Charles River (c/o D. Levy) N/A Chemicals, peptides, and recombinant proteins β-Estradiol Sigma-Aldrich Cat# E2758 Alt-R S.p. .. Cas9 Nuclease V3 IDT Cat# 1081058 Alt-R Cas9 Electroporation Enhancer IDT Cat# 1075915 (Z)-4-Hydroxytamoxifen Sigma-Aldrich Cat# H7904 Recombinant Mouse GM-CSF (carrier free) BioLegend Cat# 576304 Recombinant Mouse IL-4 (carrier free) BioLegend Cat# 574304 Collagenase D from Clostridium histolyticum Roche Cat# 11088858001 Pronase from Streptomyces griseus Millipore Sigma Cat# 10165921001 Amphotericin B solution Millipore Sigma Cat# A2942 Liberase TL Roche Cat# 5401020001 Bovine Serum Albumin, Powder Millipore Sigma Cat# A9647 Deoxyribonuclease I (DNase I) from bovine pancreas Roche Cat# 11284932001 RBC Lysis Buffer BioLegend Cat# 420301 Poly(I:C) high molecular weight InvivoGen Cat# tlrl-pic CpG-A/ODN 2216 InvivoGen Cat# tlrl-2216-1 DOTAP chloride MedChemExpress Cat# HY-112754A Concanavalin A from Canavalia ensiformis Millipore Sigma Cat# L7647, C2010 Paraformaldehyde Aqueous Solution, EM Grade Electron Microscopy Sciences Cat# 15710 Triton X-100 Thermo Fisher Scientific Cat# 85111 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific Cat# 89900 Halt Protease Inhibitor Cocktail Thermo Fisher Scientific Cat# 78429 Halt Phosphatase Inhibitor Cocktail Thermo Fisher Scientific Cat# 78420 NuPAGE LDS Sample Buffer Thermo Fisher Scientific Cat# NP0007 NuPAGE Sample Reducing Agent Thermo Fisher Scientific Cat# NP0009 TRIzol Reagent Themo Fisher Scientific Cat# 15596018 Digitonin Thermo Fisher Scientific Cat# BN2006 Trizma Hydrochloride Solution, pH 7.4 Millipore Sigma Cat# T2194 Sodium Chloride Solution, 5 M Millipore Sigma Cat# 59222C Magnesium Chloride Solution, 1M Millipore Sigma Cat# M1028 IGEPAL CA-630 Millipore Sigma Cat# I8896 Protector RNase inhibitor Millipore Sigma Cat# 3335402001 DL-Dithiothreitol (DTT) solution, 1M Millipore Sigma Cat# 646563 Bovine Serum Albumin Fraction V Solution Thermo Fisher Scientific Cat# 15260037 10% Tween 20 Bio-Rad Cat# 1662404 Critical commercial assays Venor GeM Mycoplasma PCR-Based Detection Kit Sigma-Aldrich Cat# MP0025 SF Cell Line 4D-Nucleofector X Kit L Lonza Cat# V4XC-2012 KT Streptavidin microbeads Miltenyi Biotec Cat# 130-048-101 LD columns Miltenyi Biotec Cat# 130-042-901 (Continued on next page) Immunity 59, 270–287.e1–e11, February 10, 2026 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Combined snRNA-Seq and snATAC-Seq primary splenic pDCs Pucella et al. 72 GEO: GSE252189 Experimental models: Cell lines Mouse: L929 ATCC Cat# CCL-1, RRID:CVCL_0462 Mouse: B16-Flt3L Mach et al. 82 N/A Mouse: HoxB8-FL Redecke et al. 83 N/A Mouse: Pmi28 c/o C. Cardoso N/A African green monkey: Vero E6 ATCC Cat# CRL1586; RRID: CVCL_0574 Experimental models: Organisms/strains Mouse: Smc3 fl : B6(SJL)Smc3 tm1c(EUCOMM)Wtsi /J The Jackson Laboratory RRID: IMSR_JAX:030559 Mouse: Rosa26 CreERT2 Dr. Thomas Ludwig, Ohio State University N/A Mouse: Ifn ΔTB2 This paper N/A Mouse: Ifn ΔTB2/TB4 This paper N/A Mouse: Ifn ΔTB2/TB3/TB4 This paper N/A Mouse: Irf8 -/- : B6(Cg)-Irf8 tm1.2Hm /J The Jackson Laboratory RRID:IMSR_JAX:018298 Mouse: Ctrl for snRNA-Seq/snATAC-Seq: C57BL/6-Tg(CAG-EGFP)131Osb/LeySopJ The Jackson Laboratory RRID:IMSR_JAX:006567 Mouse: C57BL/6: C57BL/6NTac Taconic Biosciences Model# B6-F Mouse: BALB/c: BALB/cAnNTac Taconic Biosciences Model# BALB-M Oligonucleotides sgRNAs targeting IFN-I TB in mouse germline, see Table S6 This paper N/A sgRNAs for targeting in HoxB8-FL cells, see Table S6 This paper N/A Primers for genotyping of Ifn ΔTB2 , Ifn ΔTB3 , and Ifn ΔTB4 alleles, see Table S6 This paper N/A RT-qPCR primers for Ifn subtype quantification, see Table S6 This paper N/A Alt-R CRISPR-Cas9 Negative Control crRNA #1 IDT Cat# 1072544 Alt-R CRISPR-Cas9 tracrRNA IDT Cat# 1072532 BAC clones RP23-64G11, RP23-139P14 and RP23-360M23 BACPAC Resources N/A Software and algorithms FlowJo v10 Tree Star RRID: SCR_008520 GraphPad Prism 10 Graphpad Inc RRID: SCR_002798 ExAtlas https://kolab.elixirgensci.com/exatlas/ N/A Heatmapper http://www.heatmapper.ca/expression/ RRID:SCR_016974 IGV https://igv.org/ RRID:SCR_011793 Resgen https://resgen.io/ N/A ImageJ https://imagej.net/ij/ RRID:SCR_003070 bcl2fastq Illumina RRID:SCR_015058 Seq-N-Slide https://igordot.github.io/sns/ RRID:SCR_021752 Trimmomatic http://www.usadellab.org/cms/ ?page=trimmomatic RRID:SCR_011848 STAR aligner https://github.com/alexdobin/STAR RRID:SCR_004463 featureCounts https://subread.sourceforge.net/ featureCounts.html RRID:SCR_012919 DESeq2 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html RRID:SCR_015687 (Continued on next page) Immunity 59, 270–287.e1–e11, February 10, 2026 e4
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